Journal: Frontiers in Neuroanatomy

Article Title: Neural Progenitors in the Developing Neocortex of the Northern Tree Shrew ( Tupaia belangeri ) Show a Closer Relationship to Gyrencephalic Primates Than to Lissencephalic Rodents

doi: 10.3389/fnana.2018.00029

Figure Lengend Snippet: Pax6 and Tbr2 expression in the germinal zones of the developing tree shrew neocortex. (A–D) Double-Immunofluorescence for Pax6 (red) and Tbr2 (green) and DAPI staining (blue) on 30 μm-cryosections of E32–P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 μm. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. (E,F) Quantification of Pax6+/Tbr2–, Pax6+/Tbr2+ and Pax6–/Tbr2+ NPCs in the VZ (E) and SVZ (F) of the E32–P1 tree shrew neocortex, expressed as number of cells per 200 μm ventricular surface. Color legend is shown in (F) . (G) Quantification of Pax6+ (Pax6+/Tbr2–, Pax6+/Tbr2+) NPCs (G) and Tbr2+ (Pax6–/Tbr2+, Pax6+/Tbr2+) NPCs (H) in the VZ and SVZ of the E32–P1 tree shrew neocortex, expressed as percentage of the sum of VZ and SVZ. Color legend is shown in (H) . (I,J) Quantification of Pax6+ (Pax6+/Tbr2–, Pax6+/Tbr2+) NPCs (I) and Tbr2+ (Pax6–/Tbr2+, Pax6+/Tbr2+) NPCs (J) in the iSVZ and oSVZ of the E32–P1 tree shrew neocortex, expressed as percentage of the sum of iSVZ and oSVZ. Color legend is shown in (J) . (E–J) Cortical wall corresponding to a total ventricular surface of 4.12–12.081 mm was analyzed. Data represent mean ± SD and are from two brains each.

Article Snippet: The following primary antibodies were used: Tbr1 (1:200, rabbit, Millipore, AB 10554), Pax6 (1:100, rabbit, Biozol, BLD 901301), Tbr2 (1:100, sheep, R&D Systems, AF61669), PCNA (1:100, mouse, Novus Biologicals, NB500-106), phosphorylated vimentin (pVim, 1:100, mouse, Abcam, ab22651), Par3 (1:200, rabbit, Millipore, 07-330), ZO-1 (1:200, mouse, Invitrogen, 33-9100), Tau1 (1:100, mouse, Millipore, MAB3420), Brn2 (1:100, mouse, Santa Cruz, sc-393324).

Techniques: Expressing, Immunofluorescence, Staining

Journal: Frontiers in Neuroanatomy

Article Title: Neural Progenitors in the Developing Neocortex of the Northern Tree Shrew ( Tupaia belangeri ) Show a Closer Relationship to Gyrencephalic Primates Than to Lissencephalic Rodents

doi: 10.3389/fnana.2018.00029

Figure Lengend Snippet: Neural progenitor subtypes in the developing tree shrew neocortex. (A–F) Triple-immunofluorescence for phospho-vimentin (pVim, white), Pax6 (red), and Tbr2 (green) and DAPI staining (blue) on 30 μm-cryosections of E37 tree shrew neocortex. The merge images show combined immunofluorescence for pVim, Pax6, and Tbr2. Open arrowheads, cell bodies; solid arrowheads, pVim+ processes. Scale bars, 20 μm. VZ, ventricular zone; SVZ, subventricular zone. (G–I) Quantification of the distinct progenitor cell types at M-phase, identified by their location of mitosis and morphology at M-phase, in the E37 tree shrew neocortex, expressed as the percentage of total pVim+ cells. Data are the sum of two brains. The total number of pVim+ mitoses quantified was 642. (J–M) Quantification of the distinct progenitor cell types at M-phase, identified by pVim staining, that are Pax6+/Tbr2– (red), Pax6+/Tbr2+ (red/green), Pax6–/Tbr2+ (green), or Pax6–/Tbr2– (white) in the E37 tree shrew neocortex. Data are the sum of two brains. The total number of pVim+ mitoses analyzed was as in (G–I) . (G–M) AP, apical progenitor; BP, basal progenitor; bIP, basal intermediate progenitor; bRG, basal radial glia, bTG; basal tangential glia; -P, process.

Article Snippet: The following primary antibodies were used: Tbr1 (1:200, rabbit, Millipore, AB 10554), Pax6 (1:100, rabbit, Biozol, BLD 901301), Tbr2 (1:100, sheep, R&D Systems, AF61669), PCNA (1:100, mouse, Novus Biologicals, NB500-106), phosphorylated vimentin (pVim, 1:100, mouse, Abcam, ab22651), Par3 (1:200, rabbit, Millipore, 07-330), ZO-1 (1:200, mouse, Invitrogen, 33-9100), Tau1 (1:100, mouse, Millipore, MAB3420), Brn2 (1:100, mouse, Santa Cruz, sc-393324).

Techniques: Immunofluorescence, Staining

Journal: Frontiers in Neuroanatomy

Article Title: Neural Progenitors in the Developing Neocortex of the Northern Tree Shrew ( Tupaia belangeri ) Show a Closer Relationship to Gyrencephalic Primates Than to Lissencephalic Rodents

doi: 10.3389/fnana.2018.00029

Figure Lengend Snippet: List of references of neurodevelopmental parameters used for statistical analysis.

Article Snippet: The following primary antibodies were used: Tbr1 (1:200, rabbit, Millipore, AB 10554), Pax6 (1:100, rabbit, Biozol, BLD 901301), Tbr2 (1:100, sheep, R&D Systems, AF61669), PCNA (1:100, mouse, Novus Biologicals, NB500-106), phosphorylated vimentin (pVim, 1:100, mouse, Abcam, ab22651), Par3 (1:200, rabbit, Millipore, 07-330), ZO-1 (1:200, mouse, Invitrogen, 33-9100), Tau1 (1:100, mouse, Millipore, MAB3420), Brn2 (1:100, mouse, Santa Cruz, sc-393324).

Techniques:

Journal: Frontiers in Neuroanatomy

Article Title: Neural Progenitors in the Developing Neocortex of the Northern Tree Shrew ( Tupaia belangeri ) Show a Closer Relationship to Gyrencephalic Primates Than to Lissencephalic Rodents

doi: 10.3389/fnana.2018.00029

Figure Lengend Snippet: Untransformed values of neurodevelopmental parameters of tree shrew, macaque, rat/mouse and ferret used for statistical analysis.

Article Snippet: The following primary antibodies were used: Tbr1 (1:200, rabbit, Millipore, AB 10554), Pax6 (1:100, rabbit, Biozol, BLD 901301), Tbr2 (1:100, sheep, R&D Systems, AF61669), PCNA (1:100, mouse, Novus Biologicals, NB500-106), phosphorylated vimentin (pVim, 1:100, mouse, Abcam, ab22651), Par3 (1:200, rabbit, Millipore, 07-330), ZO-1 (1:200, mouse, Invitrogen, 33-9100), Tau1 (1:100, mouse, Millipore, MAB3420), Brn2 (1:100, mouse, Santa Cruz, sc-393324).

Techniques: